Rossi, who was leading the league scoring charts before suffering a knee ligament injury in early January, from which he is still recovering, has always had the backing of the 56-year-old coach, who appeared to leave the door open to the Fiorentina striker.
However, Prandelli said Rossi's World Cup future could depend entirely on the outcome of scheduled fitness tests.
"On Rossi I can't say anything. The tests he undergoes must be analysed and then compared with the old data we have on him," added Prandelli.
"I don't want to put any pressure on him. He needs peace to recover."
Rossi is expected to make the trip to Brazil alongside number one forward pick Mario Balotelli.
But younger, emerging talents like Mattia Destro of Roma and Torino's Ciro Immobile, who tops Serie A scoring on 19 goals -- one more than Carlos Tevez -- are also in the reckoning.
"We 'provoked' a number of younger players recently, they've silenced their critics and reacted positively," said Prandelli.
"There's no truth to the suggestion that they both can't be in Brazil."
The Azzurri coach, meanwhile, sent a clear message to the players whose bad attitude, he says, does not belong in this summer's showpiece.
"This morning I met with the players and I reminded them that the league's code of ethics will count.
"Even in the last month of the season, whoever slips up stays at home because it means he won't be able to handle the pressure of the World Cup."