Aston Villa striker Libor Kozak has been ruled out for the rest of the season after suffering a broken leg.
Kozak sustained the injury during a training session with the Premier League club, who confirmed on Thursday that the Czech Republic international would miss the remainder of the campaign.
The 24-year-old joined Paul Lambert's side from Lazio in a $11.5 million deal in pre-season and had scored four goals in 15 appearances for Villa this season.
"The Club can confirm that Libor Kozak suffered a broken leg today in training," a statement on Villa's website revealed. "He will undergo surgery tomorrow (Friday) and, regretfully, he will play no further part this season."
Four-time champions Italy secured qualification for the 2014 World Cup after overturning a first-half deficit against the Czech Republic to prevail 2-1 on Tuesday.
AC Milan striker Mario Balotelli scored the decisive goal from the penalty spot in the 54th minute after Giorgio Chiellini's header two minutes earlier had levelled Libor Kozak's superb opener for Michal Bilak's visitors, who finished the game with 10 men.
Balotelli, loved and hated in equal measure by Italy's fans, had finished the first half with arguably more detractors added to his list before making amends just before the hour mark.
With home goalkeeper Gianluigi Buffon equalling Fabio Cannavaro's all-time national record of 136 caps, there was a party atmosphere at his home Juventus stadium.
But a reality check came in the 19th minute when Kozak sent a superb first-time half-volley past Buffon after Petr Jiracek ran unchallenged down the left flank to whip in a perfect cross for the big striker.
Italy's unexpected half-time deficit was made more dramatic given their early domination, and Balotelli's failure to convert several clear-cut chances.
Balotelli collected Marek Suchy's poor clearance after Christian Maggio's timely intervention, but saw his strike from 20 yards parried by an alert Petr Cech.
Five minutes later Balotelli hit the crossbar from six yards out after the ball fell at his feet following a deep cross from the left.
The Milan striker's woes continued when he snatched a rebound from the hands of Cech six yards out only to sky his effort over the bar, and missed a more difficult chance two minutes later from Daniele De Rossi's flick-on.
Balotelli appeared to vent his frustration by stamping on defender Tomas Sivok on the half hour, and was promptly cautioned and he missed another golden chance to draw Italy level, but this time the hands of Cech were a match for the striker's close-range header following Andrea Pirlo's superb cross-field ball to the back post.
Before the interval, Antonio Candreva was unlucky to see his long-range drive tipped wide by an alert Cech.
But Italy were lucky not to concede a second as David Limbersky's dummy from a cross deep on the right allowed Kozak a first-time shot which sailed just over Buffon's crossbar on the stroke of half-time.
Prandelli kept faith with Balotelli at the start of the second half, having replaced deep-lying forward Emanuele Giaccherini with Southampton's Pablo Osvaldo and seconds after kick-off he tested Cech with a long-range effort.
Moments later Italy levelled, Juventus defender Chiellini rising to meet Candreva's corner to score in his home stadium.
Italy's World Cup hopes then increased significantly when Balotelli won a penalty following a crude challenge by Theodor Gebre Selassie and promptly stepped up to coolly beat Cech at the keeper's right post.
Italy, however, were kept on their toes and almost paid for slack defending just after the hour when Ondrej Banek's angled shot from the left crept past Buffon's far post as the goalkeeper looked on.
Two minutes later Buffon got down low to smother after Jiracek held off the Italian defence to unleash from 15 yards out.
Czech second-half substitute Daniel Kolar saw red just before full-time after receiving a second yellow card.
Aston Villa bolstered their forward line by signing Czech Republic striker Libor Kozak from Lazio on transfer deadline day.
Kozak agreed a four-year contract with the Premier League club after coming to the attention of Villa manager Paul Lambert with a series of fine displays for the Italian side in last season's Europa League.
The 24-year-old top-scored in Europe's second tier competition with 10 goals and Lambert admitted he had been tracking the player for some while.
"We've been on this one for the last few months, trying to bring him to the club, so I'm delighted to get it across the line," Lambert told Villa's website. "Libor has an unbelievable scoring record in the Europa League, incredible really. He wasn't given much opportunity for Lazio in Serie A but he showed proper potential in leading the line there at a strong club with a great tradition and we need somebody like him as we only have Christian Benteke really as a main target man."
Kozak joined Lazio from Slezsky FC Oprava in 2008 and was soon loaned out to Brescia for whom he scored four goals in 22 appearances. He eventually made 77 appearances for Lazio, scoring 22 goals, and has also won four caps for the Czechs, scoring once in a friendly against Hungary last month.
"I'm really happy to be at Aston Villa and I'm ready for this new step in my career," he said. "I hope it's a good step for me and also for Villa because everything is about the team. "This is a big club, a very historic club with a fantastic fanbase and, for me, this is really important."